Mouse anti-human TERT antibody, clone 54-2
The Mouse anti-human TERT antibody is an IgG1 monoclonal antibody designed to detect the human Telomerase Reverse Transcriptase (TERT) protein. TERT is an essential component of the telomerase enzyme, which adds telomeric repeats to chromosome ends, playing a vital role in cellular immortality and cancer progression. This antibody is versatile and can be used in various research applications, including Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IFA), immunohistochemistry (IHC), and ELISA. Researchers studying telomerase activity, cancer, stem cell biology, or age-related diseases can utilize this antibody for reliable and validated results across multiple experimental platforms.
Clone name:
54-2
Concentration:
1 mg/mL
Reactivity:
Human
Specificity:
TERT
Isotype:
IgG1
Conjugation:
Unconjugated
Form:
Liquid
Storage buffer:
Phosphate Buffered Saline containing 0.03% Proclin 300, pH 7.4.
Stability & Storage:
Store at 4°C for two weeks.
Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Application:
ELISA、WB、IFA
Product Note:
Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:50-500
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Shipping Conditions:
Blue ice
54-2
Concentration:
1 mg/mL
Reactivity:
Human
Specificity:
TERT
Isotype:
IgG1
Conjugation:
Unconjugated
Form:
Liquid
Storage buffer:
Phosphate Buffered Saline containing 0.03% Proclin 300, pH 7.4.
Stability & Storage:
Store at 4°C for two weeks.
Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Application:
ELISA、WB、IFA
Product Note:
Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:50-500
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Shipping Conditions:
Blue ice
Indirect ELISA Western Blot
IFA
293T cells were transfected with TERT plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with Anti-TERT Antibody [Clone 54-2] (1:50) followed by secondary antibodies (goat anti-Mouse IgG-iFluor488, 1:400, green) and cell nuclei were stained with DAPI (Blue).
IFA
293T cells were transfected with TERT plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with Anti-TERT Antibody [Clone 54-2] (1:50) followed by secondary antibodies (goat anti-Mouse IgG-iFluor488, 1:400, green) and cell nuclei were stained with DAPI (Blue).
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